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This paper presents new stable carbon (δ13C) and nitrogen (δ15N) isotope data obtained from human and animal remains from the Kamennyi Ambar 5 cemetery (KA-5) (Southeastern Urals, Russian Federation) and represents one of the largest stable isotope datasets from a single prehistoric site in the steppes of Central Eurasia. These results are compared with other regional faunal, botanical and archaeological datasets to examine late prehistoric trends in pastoralism and human dietary patterns. The findings of this research emphasize a subsistence regime consisting of broad-spectrum resources that include domestic and wild animal species, wild plants and fish. This study contributes to current knowledge regarding the diversity in isotopic values of human and animal remains and indicates that variation in subsistence was related to distinct local resource biomes and economic strategies. These results suggest a more complex model of late prehistoric subsistence trends in the steppes that emphasizes the need for enhanced micro-regional studies combining environmental, biological, and archaeological datasets. The study presented here also provides information on the most detailed bioarchaeological study of human remains and stable isotopes to date related to the Sintashta archaeological pattern.